Method for highly sensitive dna methylation analysis

ABSTRACT

Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.16/491,278 filed Sep. 5, 2019, which is a national phase applicationunder 35 U.S.C. § 371 of International Application No. PCT/US2018/021591filed Mar. 8, 2018, which claims the benefit of priority of U.S.Provisional Patent Application No. 62/468,907 filed Mar. 8, 2017, all ofwhich are hereby incorporated by reference in their entirety.

The invention was made with government support under Grant No. AR048177,awarded by the National Institutes of Health. The government has certainrights in the invention.

BACKGROUND OF THE INVENTION 1. Field of the Invention

This invention relates generally to molecular biology. Morespecifically, it concerns in some embodiments, compositions and methodsfor quantitative or single-base resolution analysis for DNA methylation(5-methylcytosine, 5mC) in limited biological samples including DNA fromlow cell number or a single cell, and cell-free DNA (cfDNA) from bloodor other body fluid samples, using DNMT1 coupled with stranddisplacement DNA polymerases to preserve 5mC information during wholegenome amplification process or during amplification of specific DNAfragments or loci prior to detection by quantitative PCR orhigh-throughput sequencing.

2. Background Art

Genomic methylation of cytosine bases within cytosine-guanine (CpG)dinucleotides is a crucial epigenetic mechanism implicated in theregulation of cell type-specific transcriptional patterns. Consequently,the mapping of DNA methylome landscapes at single-base resolutionprovides a number of powerful insights as to the involvement ofepigenetic regulation in development and disease. Due to the oftennotable extent of divergence between the methylation profiles of cellswithin a given population, information derived from bulk tissue samplesmay fail to account for epigenomic tissue heterogeneity caused bycell-to-cell variation; thus, bulk data could mask the differentialcontributions to biochemical processes that are achieved by individualcells. The ability to carry out single-cell whole-methylome analysiswith high rates of accuracy and CpG coverage would therefore enabledeconvolution of bulk samples for more in-depth study of a diverse arrayof biological activities in which DNA methylation plays a role,including but not limited to early embryonic development and cellulardifferentiation, the generation of induced pluripotent stem cells,cancer progression and metastasis, immunological host-defense responses,and neuronal plasticity.

On the other hand, the discovery of circulating cfDNA in the plasmaoriginating from tumor in cancer patients has opened new avenues forblood-based non-invasive test for cancer detection. Yet due to thedegraded small size and low concentration of cfDNA extractable from theplasma, the characterization of 5mC patterns in cfDNA has beentechnologically demanding. Therefore, a methylome determination methodthat is scalable with limited cell numbers or even at the single-celllevel is additionally useful for the characterization of small or rarecell populations and minute samples of DNA such as picogram-levelamounts of cfDNA as well as genomic DNA from biopsy or patients samples.The current approach provides a way to faithfully amplify the methylomefrom genomic DNA or cfDNA.

The most widely used technique currently employed for mapping genomicmethylation is whole-genome bisulfite sequencing, which providessingle-base resolution of cytosine methylation with a breadth ofcoverage encompassing over 90% of the nearly 29 million CpG sites in thehuman genome. However, while traditional bisulfite treatment proceduresenable efficient methylation mapping, they concomitantly instigate thewidespread degradation of the sample of interest and thus requiresmicrogram quantities of input DNA. As a result, standard whole-genomebisulfite sequencing can hardly be scaled to the level of small numberof cells or for single-cell analysis. For instance, Lorthongpanich et.al. (2013) refrained from bisulfite treatment altogether, insteadcombining methylation-specific restriction enzymes with quantitativePCR. While this technique enables the measurement of DNA methylation insingle cells, its applicability is limited to several dozen candidateCpGs. A more potent method that amalgamates restriction enzyme treatmentwith bisulfite sequencing, known as reduced representation bisulfitesequencing (RRBS), was demonstrated by Guo et. al. (2013) in singlecells. Such a workflow, though holding more promise, nonethelessdemonstrates a limited coverage of 0.5 to 1 million CpG sites. Recently,Smallwood et. al. (2014) adapted for single-cell analysis a modifiedbisulfite sequencing protocol using post-bisulfite adaptor tagging,extending it with a pre-amplification step, ultimately achievingcoverage of approximately 20% of CpGs per individual cell. While the CpGcoverage obtained with the reported method could be enhanced to up to48.4%, this required both the use of deep sequencing—which results inhigh PCR duplicate rates—and the combination of sequence data obtainedfrom the genomic DNA of two distinct cells. Moreover, a reliableprotocol for thorough and facile whole-genome methylation analysis ofpicogram-level samples of cell-free DNA has not yet been established inthe literature. The ability to amplify genomic DNA or cfDNA whilepreserving its methylation status is therefore critical to performingepigenomic studies involving single cells or minute starting DNAquantities.

SUMMARY OF THE INVENTION

Kits, compositions, and methods are provided specifically designed toaddress the problem of low amount of starting material by utilizing anamplification step to enlarge the limited Amplification of the DNAsamples with 5mC information retained is a key aspect. As shown in theExamples, in some embodiments the use of DNA methyltransferases alongwith strand displacement DNA polymerases to achieve simultaneous DNAamplification and methylation of the amplified hemi-methylated productsproduces fully methylated products.

Furthermore, the wide and various applicability of these embodiments ondifferent biological samples with limited amount, including low amountof purified human genomic DNA, DNA from low number or a single humancell, and cell-free DNA purified from human blood or other body fluidsis shown herein. Respective protocols for initial treatment andconditions adjusted to these different samples are developed inaccordance.

In other embodiments, the following is provided: a method of amplifyingthe target DNA molecules with retained 5mC information comprising: astep of denaturing the target DNA molecule to remove secondarystructures, a whole genome amplification step coupled with DNAmethyltransferase adding 5mC to the semi-methylated products while theyare being produced, a step of purifying the product to extract theamplified DNA with 5mC information faithfully copied from the startingmaterial.

Other embodiments include a method of amplifying 5mC DNA as described inthe preceding paragraph, and followed by sonication of the product DNA,and enrichment of DNA fragments using either a 5mC antibody or aMethyl-CpG binding protein, for example using MethylMiner™ MethylatedDNA Enrichment Kit from ThermoFisher, and analysis by downstream qPCR orhigh-throughput sequencing.

In another embodiment there is a method of amplifying 5mC DNA asdescribed in paragraph [0009], and followed by bisulfite treatment andsubsequent purification, for example using EZ DNA Methylation-Direct™Kit from Zymo, and analysis by either PCR using gene specific primersand Sanger sequencing to investigate the methylation status of anindividual gene, or bisulfite library construction and high-throughputsequencing, for example using TruSeq DNA Methylation Kit from Illumina.

In another embodiment, there is a method of amplifying 5mC DNA asdescribed in paragraph [0009], preceded by a step of extracting a singlecell or small number of cells from a sample containing a cellpopulation, and a cell lysis step of breaking the cell membrane toexpose the DNA content for direct downstream amplification.

In yet another embodiment there is a method of amplifying 5mC DNA asdescribed in paragraph [0009], preceded by a step of extracting cfDNAfrom a sample of human blood or other body fluids, quantification andcalculation of exact enzyme ratio and amount to use prior toamplification steps.

In yet another embodiment, there is a method of amplifying 5mC DNA asdescribed in paragraph [0009] to which a primase is further incorporatedduring the amplification step. The primase has a potent activity tosynthesize DNA primers. An example of a primase is Thermus thermophilus(Tth) PrimPol. In embodiments, the primase lowers the bias that may beintroducing during the priming step and enables near-complete wholegenome amplification.

In addition, there are methods, apart from the whole genomeamplification, in which the 5mC-retained system is applied to amplifyspecific loci with corresponding primers and and to acquire themethylation information of a particular fragment. This is particularlyimportant to amplify specific loci with heavy methylation. In certainembodiments. the amplified products versus a control can be detectedwith simple detection methods, such as ELISA for example. Applicationsinclude newborn screens or other screens involving loci-specificmethylation in certain diseases.

In yet another embodiment, there is a method of amplifying 5mC DNAsimilar as described in paragraph [0009] with the addition ofcorresponding primers to the specific regions of interest, preceded by astep of extracting genomic DNA from a sample of human tissue, blood orother body fluids, and followed by purification and quantification ofmethylation level, for example using Enzyme-Linked ImmunoSorbent Assay(ELISA).

There are methods that involve physically processing or manipulatingmethylated DNA in order to evaluate or analyze specific regions or on agenome-wide level. In some embodiments, a method may involve, mayinvolve at least, or may involve at most 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12 or more of the following steps (or any range derivable therein):obtaining a biological sample, isolating DNA from a biological sample,processing a biological sample to obtain DNA from the sample; denaturingDNA; exposing DNA to alkaline conditions; separating DNA from othernucleic acids; separating methylated DNA from unmethylated DNA,separating genomic DNA from non-genomic DNA, enriching for or poolingmethylated DNA or nucleic acids; isolating methylated DNA, purifyingmethylated DNA; discarding or not using nonmethylated DNA or nucleicacids other than genomic DNA; incubating methylated DNA with one or morenucleic acid probes or primers; incubating methylated DNA with apolymerase; incubating methylated DNA with a strand displacementpolymerase; selecting methylated DNA; selecting methylated DNA andincubating the selected methylated DNA with a strand displacementpolymerase; incubated methylated DNA with a polymerase under conditionsthat allow polymerization; incubating methylated DNA with amethyltransferase; incubating methylated DNA with a methyltransferaseunder conditions that allow methylation of DNA, particularly replicatedunmethylated or hemi-methylated DNA; producing or reproducing methylatedDNA molecules; isolating or purifying produced or reproduced methylatedDNA molecules; pooling produced or reproduced methylated DNA molecules,quantifying methylated DNA or methylation of DNA; comparing methylatedDNA or methylation of DNA; qualifying methylated DNA or methylation ofDNA; and/or, measuring methylation of DNA or identifying methylatednucleotides or regions. It is specifically contemplated that one or moreof these steps may be excluded as part of an embodiment.

In further embodiments, amplified or enriched or produced methylated DNAmay be subsequently treated or manipulated using 1, 2, 3, 4, 5, 6, 7, 8or more of the following steps: denaturing, hybridizing, fragmenting,cutting, sequencing, cloning, inserting into a plasmid or other vector,ligating, fixing, attaching, or conjugating. Linkers, primers and probesmay be involved though methods are not limited as such. It isspecifically contemplated that one or more of these steps may beexcluded as part of an embodiment.

In certain embodiments, methylated DNA is exposed or incubated with oneor more of the following reagents: polymerase, strand displacementpolymerase, Phi29, a Phi29 analog or homolog, methyltransferase, DNMT1or a related family member, analog or homolog, dNTPs, S-adenosinemethionine (SAM), ligase, a nucleic acid primer or set of primers, anucleic acid probe, nucleic acid buffer, polymerase buffer,methyltransferase buffer, restriction enzyme, methylation-specificrestriction enzyme, or restriction enzyme buffer. It is specificallycontemplated that one or more of these reagents may be excluded as partof an embodiment.

In some embodiments, there is a method for amplifying targetedmethylated genomic DNA comprising: a) denaturing targeted methylatedgenomic DNA; b) incubating the targeted methylated genomic DNA with apolymerase and methyltransferase under conditions to reproduce thetargeted methylated genomic DNA and create amplified genomic methylatedDNA molecules; and, c) isolating the amplified genomic methylated DNAmolecules. It is contemplated that the methylated DNA may be amplifiedor enriched by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52,53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88,89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120, 130, 140,150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280,290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420,430, 440, 441, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550,560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690,700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830,840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970,980, 990, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900,2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 31,3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, 4000, 4100, 4200, 4300,4400, 4500, 4600, 4700, 4800, 4900, 5000, 6000, 7000, 8000, 9000, 10000times (or any range derivable therein). The end DNA products may be thismuch more as far as number of molecules or by weight. It is contemplatedthat the end product may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13,14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31,32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67,68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85,86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120,130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260,270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400,410, 420, 430, 440, 441, 450, 460, 470, 480, 490, 500, 510, 520, 530,540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670,680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810,820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950,960, 970, 980, 990, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700,1800, 1900, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900,3000, 31, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, 4000, 4100,4200, 4300, 4400, 4500, 4600, 4700, 4800, 4900, 5000, 6000, 7000, 8000,9000, 10000 μg or mg or μM or mM in amount.

In another embodiments, there is a method for enriching methylatedgenomic DNA comprising: a) denaturing methylated genomic DNA; b)incubating the methylated genomic DNA with a polymerase andmethyltransferase under conditions to reproduce the methylated genomicDNA and create amplified genomic methylated DNA molecules; and, c)enriching for the amplified genomic methylated DNA molecules using anantibody or binding protein that specifically binds methylated DNA.

In yet another embodiment, there is a method for analyzing a targetedmethylated genomic sequence comprising: a) denaturing methylated genomicDNA comprising the targeted sequence; b) incubating the targetedmethylated genomic DNA with a polymerase and methyltransferase underconditions to reproduce the targeted methylated genomic sequence; c)amplifying and/or sequencing the targeted methylated genomic sequence.

Other embodiments include a method for analyzing methylated genomic DNAfrom at least one cell in a biological sample from a patient comprising:a) extracting and denaturing methylated genomic DNA from the at leastone cell; b) incubating the extracted methylated genomic DNA with apolymerase and methyltransferase under conditions to reproduce theextracted methylated genomic DNA and create amplified genomic methylatedDNA molecules, wherein the polymerase and methyltransferase are presentat a ratio between about 1:5 units to about 1:20 units; and, c)isolating the amplified genomic methylated DNA molecules.

In a further embodiment, there is a method for analyzing methylatedcell-free DNA from a cell-free biological sample from a patientcomprising: a) denaturing methylated cell-free DNA (cfDNA) from thecell-free biological sample; b) incubating the methylated cfDNA with apolymerase and methyltransferase under conditions to reproduce themethylated cfDNA and create amplified genomic methylated DNA molecules,wherein the polymerase and methyltransferase are present at a ratiobetween about 1:5 units to about 1:20 units; and, c) isolating theamplified genomic methylated DNA molecules.

In further embodiments, amplified methylated DNA is incorporated intovectors for a library or applied to an array or microarray for furtherscreening or analysis. It is contemplated that the amplified methylatedDNA may be affixed to the array or other physical support.Alternatively, the material may be applied to a well and used in one ormore subsequent reactions including sequencing. Bisulfite sequencing isparticular embodiment.

An additional embodiment includes a method for amplifying a limitedamount of targeted methylated genomic DNA comprising: a) denaturing lessthan about 1000 picograms of targeted methylated genomic DNA; b)incubating the targeted methylated genomic DNA with a polymerase andmethyltransferase under conditions to reproduce the targeted methylatedgenomic DNA and create amplified genomic methylated DNA molecules,wherein the polymerase and methyltransferase are present at a ratiobetween about 1:5 units to about 1:20 units; and, c) isolating theamplified genomic methylated DNA molecules.

In some embodiments, there is a method for amplifying methylated genomicDNA comprising: a) denaturing methylated genomic DNA; b) performingrolling circle amplification by incubating the denatured methylatedgenomic DNA with a polymerase, methyltransferase, and probe underconditions to create an amplified and methylated rolling circle product;and, c) detecting the methylation on the amplified and methylatedrolling circle products. It is specifically contemplated that the probeis a circularizable probe or a padlock or turtle probe.

The amount of enzyme included or added may be, be at least or be at mostabout 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2,0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100units (U) or micromoles (or any range derivable therein). The appliesbut is not limited to polymerase and methyltransferase. The enzyme maybe added or be in a reaction having a volume of, of at least, or of atmost about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120, 130, 140, 150,160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290,300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430,440, 441, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560,570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700,710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840,850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980,990, or 1000 microliters, milliliters, centiliters or liters (or anyrange derivable therein).

In some embodiments, the ratio of polymerase units (U) tomethyltransferase units (U) is between about 1:5 to about 1:20. Theratio of units of polymerase to methyltransferase may be, be at least,or be at most about 1:50, 1:45, 1:40, 1:35, 1:30, 1:25, 1:24, 1:23:1:22, 1:21; 1:20, 1:19, 1:18, 1:17; 1:16, 1:15, 1:14, 1;13, 1:12, 1:11,1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1,6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1,18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1 or more, or any rangederivable therein. In some embodiments the ratio of units added forpolymerase:methyltransferase is about 1:15. That ratio may also beapplied with respect to moles (pic-, nano-, or micro-) or weight (inpico-, nano- or micrograms). It is contemplated that the ratios refertemporally to when one or both enzymes are first with the substrate andunder conditions to be active. Additionally, enzyme may be added at alater point in time to increase or maintain a ratio of enzymes. In someembodiments, additional enzyme may be added that is about, is at leastabout, or is at most about 0.01×, 0.02×, 0.03×, 0.04×, 0.05×, 0.06×,0.07×, 0.08×, 0.09×, 0.1×, 0.2×, 0.3×, 0.4×, 0.5×, 0.6×, 0.7×, 0.8×,0.9×, 1.0×, 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2.0×,2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3.0×, 3.1×, 3.2×,3.3×, 3.4×, 3.5×, 3.6×, 3.7×. 3.8×, 3.9×, 4.0×, 4.1×, 4.2×, 4.3×, 4.4×,4.5×, 4.6×, 4.7×, 4.8×, 4.9×, 5.0×, 5.1×, 5.2×, 5.3×, 5.4×, 5.5×, 5.6×,5.7×, 5.8×, 5.9×, 6.0×, 6.1×, 6.2×, 6.3×, 6.4×, 6.5×, 6.6×, 6.7×, 6.8×,6.9×, 7.0×, 7.1×, 7.2×, 7.3×, 7.4×, 7.5×, 7.6×, 7.7×, 7.8×, 7.9×, 8.0×,8.1×, 8.2×, 8.3×, 8.4×, 8.5×, 8.6×, 8.7×, 8.8×, 8.9×, 9.0×, 9.1×, 9.2×,9.3×, 9.4×, 9.5×, 9.6×, 9.7×, 9.8×, 9.9×, 10.0×, 11×, 12×, 13,×, 14×,15×, 16×, 17, 18×, 19, 20×, 21×, 22×, 23×, 24×, or 25× greater than theprevious amount added or relative to the amount of another enzymepreviously added or added at that time. It is contemplated thatadditional enzyme may be added to a reaction mix within or after 10, 20,30, 40, 50, 60 minutes, or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13,14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or more hours (or any rangederivable therein). The amount added later includes any amount describedherein. Additional enzyme may be added 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ormore times (or any range derivable therein) before a reaction is haltedor stopped.

In some embodiments, the amount of genomic methylated DNA that isincubated with the polymerase and methyltransferase is between about 5picograms (pg) to about 1 microgram (m). In specific embodiments, theamount of genomic methylated DNA at the start of an enzyme reaction isabout, at least about, or at most about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46,47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64,65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240,250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380,390, 400, 410, 420, 430, 440, 441, 450, 460, 470, 480, 490, 500, 510,520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650,660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790,800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930,940, 950, 960, 970, 980, 990, 1000 picograms, nanograms, micrograms, ormilligrams or more (or any range derivable therein). Alternatively, theamount of genomic methylated DNA or total DNA in a reaction mix may beexpressed as a concentration of about, about at least or about at most0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3,0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7,1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1,3.2, 3.3, 3.4, 3.5, 3.6, 3.7. 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5,4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9,6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3,7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7,8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.5,11.0, 11.5, 12.0, 12.5, 13.0, 13.5, 14.0, 14.5, 15.0, 15.5, 16.0, 16.5,17.0, 17.5, 18.0, 18.5, 19.0. 19.5, 20.0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46,47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64,65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170,175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240,245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310,315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380,385, 390, 395, 400, 410, 420, 425, 430, 440, 441, 450, 460, 470, 475,480, 490, 500 μg/μl or pg/μl or any range derivable therein. The amountof volume added to a reaction may be about, at least about, or at mostabout 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2,0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6,1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0,3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7. 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4,4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8,5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2,7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6,8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0,10.5, 11.0, 11.5, 12.0, 12.5, 13.0, 13.5, 14.0, 14.5, 15.0, 15.5, 16.0,16.5, 17.0, 17.5, 18.0, 18.5, 19.0. 19.5, 20.0, 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160,165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230,235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300,305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370,375, 380, 385, 390, 395, 400, 410, 420, 425, 430, 440, 441, 450, 460,470, 475, 480, 490, 500, 510, 520, 525, 530, 540, 550, 560, 570, 575,580, 590, 600, 610, 620, 625, 630, 640, 650, 660, 670, 675, 680, 690,700, 710, 720, 725, 730, 740, 750, 760, 770, 775, 780, 790, 800, 810,820, 825, 830, 840, 850, 860, 870, 875, 880, 890, 900, 910, 920, 925,930, 940, 950, 960, 970, 975, 980, 990, or 1000 μl, ml, or liters or anyrange derivable therein. These volumes also apply to the amount of afluid sample from a patient, which may contain cell-free DNA.

In some embodiments, the number of cells in a sample may be verylimited. It is contemplated that no more than 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230,240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370,380, 390, 400, 410, 420, 430, 440, 441, 450, 460, 470, 480, 490, 500,510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640,650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780,790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920,930, 940, 950, 960, 970, 980, 990, or 1000 cells (or any range derivabletherein) may be involved in having their genomic DNA amplified.

In further embodiments, methods involve genomic methylated DNA that isobtained from a tissue sample, a serum sample, a blood sample, a fecalsample, a urine sample, or a cheek or mouth swab. In other embodiments,genomic methylated DNA is obtained from a biological sample that isfreshly prepared or may be prior-treated in any convenient way such asby fixation or freezing. In some cases fresh, frozen or fixed cells ortissues may be used, e.g. FFPE tissue (Formalin Fixed ParaffinEmbedded). Thus, tissue sections, treated or untreated, may be used.Alternatively a touch imprint sample of a tissue may be used, thoughtther cytological preparations may be used, e.g. cells immobilized orgrown on slides, or cells prepared for flow cytometry.

In some embodiments, nucleic acids are denaturing. Denaturing involvesalkaline denaturation in certain cases.

Methods and kits may involve one or more polymerase enzymes. In mostembodiments, the polymerase is a strand displacement polymerase. Phi29and Phi29 variants may be used as a strand displacement polymerase.Variants include those identified in US Patent Publication 20170015980,which is hereby incorporated by reference. Bst polymerase is another onethat is used.

Some embodiments of methods and kits involve a methyltransferase thatcan install 5-methylcytosine (5mC) on hemi-methylated DNA to a fullymethylated state. In certain embodiments, the methyltransferase is DNMT1(DNA (cytosine-5)-methyltransferase 1), which may be human DNMT1, or itmay be from another mammalian source, such as a mouse. Anotherembodiment might include M.SssI DNMT. US Patent application publication20140363815 provides additional information about DNMT, which isspecifically incorporated by reference.

In some aspects the kits include an enzyme comprising a primase andpolymerase function to synthesize primers in the DNA amplification step.An example of such an enzyme is a dual primase/polymerase (primpol) fromThermus thermophilus. Other enzymes such as primases are known to aperson of skill in the art.

In some cases, methods involve preparing a master mix separate from anenzyme mix comprising the polymerase and methyltransferase prior themethylated DNA with the enzymes under conditions to polymerize andmethylate nucleic acids. In other embodiments, primases are added to themaster mix to synthesize primers during the amplification step. Kits mayinclude a master mix separate from an enzyme mix. In some embodiments, apolymerase is not incubated with a methyltransferase for more than 1, 2,3, 4, 5, 6, 7, 8, 9, 10 minutes or more (or any range derivabletherein). Temperatures at which reagents, a master mix, one or moreenzymes or an enzyme mix may be maintained or kept include −70, −20, 0,1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20,21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74,75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 Centigrade or Fahrenheit for 30 seconds,1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 minutes, 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,23, 24 hours, 1, 2, 3, 4, 5, 6, 7 days or any range derivable therein.

In some embodiment a reaction is conducted at temperatures of, of atleast, or of at most 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, or 40° C. or more, though it isspecifically contemplated that temperatures may be in the range of 30°C. to 40° C., particularly at about 37° C.

In some embodiments, a reaction or enzyme mix comprises a methylcofactor. In some cases, the methyl cofactor is S-adenosyl methionine(SAM). In additional embodiments, a kit and/or master mix includesdNTPs, DNA buffer and/or magnesium or a magnesium salt. In otherembodiments, genomic methylated DNA and random primers are added to themaster mix prior to mixing the master mix with the enzyme mix. In a kit,there may be one or more probes, primers, sets of primers, which may berandom primers.

In some embodiments, methods may also involve adding more methylcofactor after more than 3 hours of the start of incubation. In someembodiments, the amount of methyl cofactor added initially or added at alater time is about, at least about, or at most about 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79,80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200microliters or centiliters of cofactor at a concentration of about 0.01,0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5,0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9,2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3,3.4, 3.5, 3.6, 3.7. 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7,4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1,6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5,7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9,9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.5, 11.0,11.5, 12.0, 12.5, 13.0, 13.5, 14.0, 14.5, 15.0, 15.5, 16.0, 16.5, 17.0,17.5, 18.0, 18.5, 19.0. 19.5, 20.0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170,175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240,245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310,315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380,385, 390, 395, 400, 410, 420, 425, 430, 440, 441, 450, 460, 470, 475,480, 490, 500 μM or mM or M (or any range derivable therein).

In additional embodiments, methods may also involve deactivating thepolymerase and/or methyltransferase.

The source of the methylated DNA is not limiting. In some embodiments,it is from a patient, who may be human or another mammal or animal. Insome cases, the biological sample may be from a human or other mammal orbird or fish or other animal, but the methylated DNA molecule is from adifference source such as a microbe. In some methods, there is anadditional step of obtaining a biological sample from the patientcomprising the genomic methylated DNA. The biological sample may be atissue sample, a blood or serum sample, fecal sample, urine sample, or acheek or mouth swab.

In some embodiments, methods comprise treating reproduced methylated DNAmolecules with bisulfite. The molecules may or may not be isolated. Insome embodiments, methylated DNA products may be sequenced. In othercases, methods may involve detecting or identifying 5-methylcytosine inthe isolated reproduced methylated DNA molecules.

In some cases, methods involve denaturing the amplified genomicmethylated DNA molecules. In other embodiments, methods includesonicating the methylated DNA molecules, which may be amplified and/orpurified or isolated. In additional cases, there is also a step ofenriching for genomic methylated DNA molecules, which may be anamplified pool of molecules and/or may be denatured. In some cases,enriching for denatured amplified genomic methylated DNA moleculesinvolves a 5mC antibody or a methyl-CpG binding protein.

One of the reasons the embodiments disclosed herein are needed is toallow for analysis of genomic methylated DNA. Embodiments includeanalyzing the enriched denatured amplified genomic methylated DNAmolecules. Methylated DNA may be analyzed or evaluated in a number ofways, including, but not limited to those set forth according to USpatent application publications 20160115525, 20140178881, 20150056616,20150056616, 20140004511, which are hereby incorporated by reference.Additionally or alternatively, other commercially available methylationquantification or detection kits/reagents may be used, such as byThermoFisher, Enzo Life Sciences, EpiGentek. Abcam, Slgman-Aldrich, Zymo(among others) or a Bioanalyxer (Agilent) may be implemented. Analyzingthe enriched denatured amplified genomic methylated DNA moleculesinclude but are not limited to these procedures or assays: quantitativePCR, sequencing, or an array-based analysis. Other examples includeusing qPCR, an array, sequencing, methylation-sensitive restrictionenzyme digestion, SMRT, high throughput sequencing, or nanopore. In someembodiments, detecting enriched methylated DNA can be accomplished withqPCR, array, sequencing (after bisulfite) or other approaches, includingbut not limited to methylation-sensitive restriction enzyme digestion(using restriction enzymes that cleave DNA at specificunmethylated-cytosine residues while leaving methylated-cytosineresidues intact, such as HpaII and MspI for 5mC detection within CCGGrecognition site); SMRT sequencing (single molecule, real timesequencing developed by Pacific Biolab using zero-mode waveguides andphospholinked nucleotides to detect DNA bases while the DNA polymeraseproducing a natural DNA strand); and nanopore sequencing (detecting theorder of DNA nucleotides while the DNA strand is controlled to passthrough a nanopore and cause changes in the ionic current levels).

In some embodiments, methods involve amplifying and/or sequencing one ormore target genomic regions using at least one pair of primers specificto the target genomic regions. In certain embodiments, the primers areheptamers. In other embodiments, enzymes are added such as primases orprimase/polymerase combination enzyme to the amplification step tosynthesize primers.

Additional embodiments concern preparing a library from the amplifiedgenomic methylated DNA molecules. In other embodiments, methods involvelysing one or more cells from a biological sample from a patient. Inother cases, methods involve extracting DNA from a biological samplefrom a patient.

Embodiments also include one or more kits comprising, in suitablecontainer(s), 1, 2, 3, 4, 5, 6, 7, 8 or more of the following: stranddisplacement polymerase, Phi29 or a variant thereof, DNMT1, human ormouse DNMT1, S-adenosine methionine, dNTPs, random primers, and one ormore nucleic acid buffers, magnesium or a magnesium salt, or BSA. Insome cases the kits include an enzyme comprising a primase andpolymerase function to synthesize primers in the DNA amplification step.An example of such an enzyme is a dual primase/polymerase (primpol) fromThermus thermophilus.

The use of the word “a” or “an” when used in conjunction with the term“comprising” in the claims and/or the specification may mean “one,” butit is also consistent with the meaning of “one or more,” “at least one,”and “one or more than one.”

It is contemplated that any embodiment discussed herein can beimplemented with respect to any method or composition of the invention,and vice versa. Furthermore, compositions and kits of the invention canbe used to achieve methods of the invention.

Throughout this application, the term “about” is used to indicate that avalue includes the standard deviation of error for the device or methodbeing employed to determine the value.

The use of the term “or” in the claims is used to mean “and/or” unlessexplicitly indicated to refer to alternatives only or the alternativesare mutually exclusive, although the disclosure supports a definitionthat refers to only alternatives and “and/or.” It is also contemplatedthat anything listed using the term “or” may also be specificallyexcluded.

As used in this specification and claim(s), the words “comprising” (andany form of comprising, such as “comprise” and “comprises”), “having”(and any form of having, such as “have” and “has”), “including” (and anyform of including, such as “includes” and “include”) or “containing”(and any form of containing, such as “contains” and “contain”) areinclusive or open-ended and do not exclude additional, unrecitedelements or method steps.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the specificexamples, while indicating specific embodiments of the invention, aregiven by way of illustration only, since various changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. A flow chart showing the processing steps of 5mC-WGA, with modelof co-functioning of Phi29 and DNMT1. As shown in the model, DNMT1 addsnew 5mC based on the template 5mC patterns at the double strand DNAregions synthesized by Phi29, so that the 5mC marks are faithfullycopied during DNA amplification. The optimal enzymatic ratio betweenPhi29 and DNMT1 ensures DNMT1 has ample time to add 5mC before doublestrands are displaced by a new working Phi29.

FIG. 2. Test regions selection and design of primers. Two genome regionswith different methylation patterns are displayed along with regions ofcomplementary primer binding (shown as arrows). NFATC1 locus is selectedas the hypermethylated test region with 20 confirmed 5mCpG sites, whileMAPK8IP2 locus is selected as the unmethylated test region with 21confirmed unmethylated CpG sites.

FIG. 3. Bisulfite treatment and Sanger sequencing data of the amplifiedtest regions. At known 5mCpG sites in the hypermethylated region (last 6sites of NFATC locus are shown), unlike WGA control group, all 5mCpGsites have been retained in the amplified product of 5mC-WGA group (bluepeaks), indicating efficient 5mC copying activity of this method. Atknown unmethylated CpG sites in the unmethylated region (last 5 sites ofMAPK8IP2 locus are shown), both WGA control and 5mC-WGA groups showcomplete C to T conversion, indicating minimal false positive de novomethylation activity of this method. Each reaction shown here utilized 5pg human brain gDNA as the template and the same reaction compositiondescribed in the preferred embodiments section, with the only exceptionthat WGA control groups use no DNMT1. Black asterisks indicate basesthat are non-CpG cytosines in the unconverted gDNA sequence; pinkasterisks denote CpG cytosines in the unconverted gDNA sequence.

FIG. 4A-B. Optimization data of 5mC-WGA reaction using different enzymeratios (a) and input DNA amounts (b). Except for the reaction componentbeing optimized, all the rest of the reaction components stay the sameas described in the preferred embodiments section. The results areevaluated using bisulfite treatment and Sanger sequencing. ‘C’ reads inthe hypermethylated locus denote accuracy of 5mC copying; while ‘T’reads in the unmethylated locus denote elimination of de nove 5mCincorporation.

FIG. 5A-B. Sonicated gDNA (a) and cell-free DNA (b) amplificationprofiles after 5mC-WGA reaction analyzed by Bioanalyzer. Purple andgreen signify upper and lower molecular markers. Main products after5mC-WGA in two groups both largely converge with or exceed the uppermarker region, indicating successful >10 kb amplification.

FIG. 6. MeDIP peak patterns at example sites identified in 5mC-WGAsample and template DNA sample. Similar patterns are observed in bothgroups, indicating efficient genome-wide 5mC copying during 5mC-WGA.

FIG. 7A-B. Global DNA methylation level in a CpG (CG) and non-CpG(CHH/G) context for 10 pg genomic DNA amplified samples and the bulksample as the positive control (a) confirmed the retainability of themethylome during the amplification. (b) Pearson correlation ofmethylated CpG sites for 10 pg genomic DNA amplified samples and controlsample B S-seq datasets. Pearson analysis of BS-seq datasets foramplified samples and the positive control demonstrated high correlationof the methylated CpG sites acquired with the high-throughputsequencing.

DETAILED DESCRIPTION OF THE EMBODIMENTS

The present disclosure relates to the methods and kits for manipulating,producing, creating, amplifying purifying, isolating, analyzing,assaying, measuring, sequencing, and evaluating methylation of nucleicacids, particularly genomic DNA. What is provided is a way to amplifyDNA from very limited biological samples (e.g. from small number ofcells, body fluids, or biopsy samples) with DNA methylation(5-methylcytosine, 5mC) information faithfully copied from the startingmaterial. A step for processing the DNA into unstructured form fromvarious biological samples while being compatible with subsequentenzymatic reactions; a step for amplifying the template DNA moleculeswhile copying the 5mC patterns of template into the product DNAmolecules; a step of purifying the enzymatic reaction and prepare theamplified products for downstream applications. An enzymatic reactionsystem comprising of salts, S-adenosyl-methionine (SAM), deoxynucleotidetriphosphates (dNTPs), random primers, and a mixture of a DNA polymerasewith strand displacement activity and a DNA methylation-maintenanceenzyme at an exact ratio.

It is generally desirable to be able sensitively, specifically,qualitatively and/or quantitatively to detect methylated DNA, and inparticular genomic DNA, in a sample, including for example in fixed orfresh cells or tissues or in a cell free biological sample. It may beparticularly desirable to detect, sequence, or evaluate methylated DNAin a single cell. For example, in population-based assays that analyzethe content of many cells, molecules in rare cells may escape detectiondue to the low abundance of material to evaluate. This is similarly truefor cell-free biological samples.

The sample may, for example, be derived from a tissue or organ of thebody, or from a bodily fluid. Such a sample will advantageously be orcomprise a cell or group of cells such as a tissue. The sample may, forexample, be a colon, lung, pancreas, prostate, skin, thyroid, liver,ovary, endometrium, kidney, brain, testis, lymphatic fluid, blood,plasma, urinary bladder, or breast sample, or comprise colon, lung,pancreas, prostate, skin, thyroid, liver, ovary, endometrium, kidney,brain, testis, lymphatic fluid, blood, urinary bladder, or breast cells,groups of cells or tissue portions. Samples may be cultured or harvestedor biopsied cell or tissue samples, e.g. as mentioned above, in whichthe methylated genomic DNA may be detected to reveal the qualitative orquantitative nature of the methylation that it is present, or thenucleotide sequence of methylated nucleic acids at one or more specificgenes, regions, CpG islands and the like. The sample of cells may befreshly prepared or may be prior-treated in any convenient way such asby fixation or freezing. Accordingly, fresh, frozen or fixed cells ortissues may be used, e.g. FFPE tissue (Formalin Fixed ParaffinEmbedded). Thus, tissue sections, treated or untreated, may be used.

A “CpG island” as used herein refers to regions of DNA with a high G/Ccontent and a high frequency of CpG dinucleotides relative to the wholegenome of an organism of interest. Also used interchangeably in the artis the term “CG island.” The ‘p’ in “CpG island” refers to thephosphodiester bond between the cytosine and guanine nucleotides.

DNA may be isolated from an organism of interest, including, but notlimited to eukaryotic organisms and prokaryotic organisms, preferablymammalian organisms, such as humans.

DNA methyltransferases (MTases) that transfer a methyl group fromS-adenosylmethionine to either adenine or cytosine residues, are foundin a wide variety of prokaryotes and eukaryotes.

In certain aspects, the step of enriching a sample for sequencescomprising CpG islands can be done in different ways. One technique forenrichment is immunoprecipitation of methylated DNA using amethyl-Cytosine specific antibody (Weber et al., 2005). Alternatively,an enrichment step can comprise digesting the sample with a one or morerestriction enzymes which more frequently cut regions of DNA comprisingno CpG islands and less frequently cut regions comprising The terms“target, “target sequence”, “target region”, and “target nucleic acid,”“target DNA” etc. are used synonymously herein and refer to the nucleicacid, or to a region or sequence thereof, which is to be detected or towhich a reagent used in the method binds, for example the methylated DNAto be detected, or more particularly the regions thereof, to which aprobe is hybridized or primers hybridize or amplify. Thus a targetsequence may be within a gene or outside a gene or in a coding region ora noncoding region. As discussed above, the methylation may be genomicDNA that includes one or more CpG islands. In some embodiments, multipleCpG islands are targeted.

The term “hybridization,” as used herein, refers to the formation of aduplex structure by two single-stranded nucleic acids due tocomplementary base pairing. Hybridization can occur between fullycomplementary nucleic acid strands or between “substantiallycomplementary” nucleic acid strands that contain minor regions ofmismatch. Conditions under which hybridization of fully complementarynucleic acid strands is strongly preferred are referred to as “stringenthybridization conditions” or “sequence-specific hybridizationconditions”. Stable duplexes of substantially complementary sequencescan be achieved under less stringent hybridization conditions; thedegree of mismatch tolerated can be controlled by suitable adjustment ofthe hybridization conditions. Those skilled in the art of nucleic acidtechnology can determine duplex stability empirically considering anumber of variables including, for example, the length and base paircomposition of the oligonucleotides, ionic strength, and incidence ofmismatched base pairs, following the guidance provided by the art (see,e.g., Sambrook et al., 1989.; Wetmur, 1991; and Owczarzy et al., 2008,which are incorporated herein by reference). Thus the design ofappropriate primers and probes, and the conditions under which theyhybridize to their respective targets is well within the routine skillof the person skilled in the art.

Methods involve a polymerase that replicates methylated genomic DNA.Strand displacement polymerase are used in some embodiments. DNApolymerase such phi29 (ϕ29) polymerase, Klenow fragment, Bacillusstearothermophilus DNA polymerase (BST), T4 DNA polymerase, T7 DNApolymerase, or DNA polymerase I may be used.

In certain aspects, methods also involve incorporation of a primase withpotent activity in the amplification step to synthesize DNA primers,such as for example a primase/polymerase from Thermus thermophilus (Tth)PrimPol, so as to lower the bias introduced during priming step andenable near-complete whole genome amplification.

In some embodiments, methods involve amplifying and/or sequencing one ormore target genomic regions using at least one pair of primers specificto the target genomic regions. In certain embodiments, the primers areheptamers. In other embodiments, enzymes are added such as primases orprimase/polymerase combination enzyme to the amplification step tosynthesize primers.

An example of an enzyme with dual activity is an enzyme with primase andpolymerase function (primpol) is obtained from the thermophilic bacteriaThermus thermophilus. The enzyme combines two distinct and complementaryactivities in a single thermo-stable protein: primase and polymerase.The enzme creates its own primer sequence.

PrimPol from T thermophilus shows a great tolerance to damaged DNA. DNAis subject to chemical modifications within the cells. PrimPol has theability to introduce a variety of substrate nucleotides (e.g.fluorescent nucleotides) into DNA and RNA template molecule.

PrimPol has a role in multiple displacement amplification (MDA)reactions, generating primers for its subsequent use by Phi29 DNApolymerase, thus making unnecessary the use of random synthetic primersand possibly resulting in a more uniform amplification of DNA.

Primases are enzymes known in the art, such as for example bacterial T7primase. T7 is used to amplify genomic DNA. However, it is has been usedwith a method involving low throughput sequencing and qPCR, and with astarting material of at least 1 ng gDNA. This is much larger volume thanwhat is taught in the current disclosure.

Rolling circle amplification is known in the art. Upon the hybridizationof the terminal regions of a padlock probe to a complementary cDNAsequence, the padlock probe is “circularized” by ligation. Thecircularization of the padlock probe(s) may be carried out by ligating,directly or indirectly, the ends of said padlock probe(s). Procedures,reagents and conditions for this are well known and described in the artand may be selected according to choice. In specific embodiments, the inthe circularization of the padlock probe(s) step, the terminal regionsof the padlock probe may hybridize to non-contiguous regions of the cDNAsuch that there is a gap between said terminal regions.

The term “library” refers to a collection (e.g., to a plurality) ofvehicles that comprise the amplified genomic methylated DNA molecules.The vehicle may be a vector, construct, array, or other physicalvehicle. A “vector” or “construct” (sometimes referred to as genedelivery or gene transfer “vehicle”) refers to a macromolecule, complexof molecules, or viral particle, comprising a polynucleotide to bedelivered to a host cell, either in vitro or in vivo. The polynucleotidecan be a linear or a circular molecule. One of skill in the art would bewell equipped to construct a vector through standard recombinanttechniques (see, for example, Maniatis et al., 1988 and Ausubel et al.,1994, both incorporated herein by reference). An array comprises a solidsupport with nucleic acid probes attached to the support. Arraystypically comprise a plurality of different nucleic acid probes that arecoupled to a surface of a substrate in different, known locations. Thesearrays, also described as “microarrays” or colloquially “chips” havebeen generally described in the art, for example, U.S. Pat. Nos.5,143,854, 5,445,934, 5,744,305, 5,677,195, 6,040,193, 5,424,186 andFodor et al., 1991), each of which is incorporated by reference in itsentirety for all purposes. Techniques for the synthesis of these arraysusing mechanical synthesis methods are described in, e.g., U.S. Pat. No.5,384,261, incorporated herein by reference in its entirety for allpurposes. Although a planar array surface is used in certain aspects,the array may be fabricated on a surface of virtually any shape or evena multiplicity of surfaces. Arrays may be nucleic acids on beads, gels,polymeric surfaces, fibers such as fiber optics, glass or any otherappropriate substrate, see U.S. Pat. Nos. 5,770,358, 5,789,162,5,708,153, 6,040,193 and 5,800,992, which are hereby incorporated intheir entirety for all purposes.

Example 1 Reaction Set-Up

This invention is directed to a method of amplifying the target DNAmolecules with retained 5mC information comprising:

-   -   (a) a step of denaturing the target DNA molecule to remove        secondary structures,    -   (b) a whole genome amplification step coupled with DNA        methyltransferase adding 5mC to the semi-methylated products        while they are being produced,    -   (c) a step of purifying the product to extract the amplified DNA        with 5mC information faithfully copied from the starting        material.

The design of the workflow is shown in FIG. 1 and describe in detailsbelow:

-   -   (a) Alkaline denaturation of genomic DNA, for example using        REPLI-g Mini Kit from Qiagen.

First prepare buffers: Prepare Buffer D1 by adding 16 ul water to 4.5 ulBuffer DLB and Buffer N1 by adding 42.5 ul water to 7.5 ul StopSolution.

Then dilute genomic brain DNA to a concentration of 200 pg/ul. Add 9 ulwater to 1 ul diluted genomic DNA (200 pg). Add 10 ul Buffer D1 togenomic DNA, vortex and briefly centrifuge the mixture, and incubate atroom temperature for 3 minutes.

Add 20 ul Buffer N1 to the denatured genomic DNA, then vortex andbriefly centrifuge the mixture.

The final DNA concentration of the solution is 5 pg/ul. For each 5-pginput reaction, add 5 ul random heptamer primer stock (for example, fromThermoFisher) to 1 ul denatured gDNA and place on ice.

-   -   (b) Whole genome amplification step coupled with DNA        methyltransferase, for example use Phi29 as polymerase and human        DNMT1 as methyltransferase, both from NEB.

First prepare a WGA master mix on ice based on the followingsingle-reaction volumes: 5 ul 10×Dnmt1 Buffer, 1.67 ul 10 mM dNTPs, 0.5ul 100×BSA, 0.5 ul 1 M MgCl2, 0.5 ul 1 M (NH4)2SO4, 17.83 ul water,totaling 26.00 ul.

Then aliquot the master mix into the appropriate number of reactions,and add 6 ul genomic DNA/primer mix to each.

Next prepare WGA enzyme mix on ice based on the followingsingle-reaction volumes: 15 ul DNMT1 [30 units]; 1.0 ul 32 mM SAM; 2 ulof 10×Phi29 dilution [2 units]. Note that do not combine the enzyme mixwith the above master mix yet, as Phi29 polymerase is maximally activeat a lower temperature than Dnmt1 and reaction coupling may be lost.

Finally finish the setup of the 5mC-WGA reaction by first equilibrateenzyme-less reaction mixes and enzyme mixes at 37° C. for 5 minutes,then add reaction mixes to corresponding enzyme mixes. Incubatereactions at 37° C. for 10 hours, adding 0.5 ul 32 mM fresh SAM to eachreaction after 5 hours. Then heat-inactivate DNMT1 and Phi29 polymerasefor 20 minutes at 65° C., and hold reactions at 4° C. after completion.

-   -   (c) Purification of the product. Using magnetic beads-based        protocol is preferred than column-based method, as the size of        the product is larger than 10 kb. For example, using Ampure XP        beads from Beckman. Adding beads with the reaction with 1:1        ratio in volume, mix well and incubate at room temperature for        15 minutes, wash with 200 ul of 80% ethanol for two times        without disturbing the beads, dry the beads at room temperature        for 5 minutes, then elute with 20 ul nuclease-free water. The        purified products are now ready to be used in the downstream        applications. The present invention will be next more        specifically described by way of Examples, which will not be        construed as limiting the invention.    -   (d) IF the system includes primases. With alkaline denaturation        and the purification remain the same, the workflow is only        modified during the amplification step with the addition of the        primase. For example, with TruePrime WGA kit, prepare a 20 ul        reaction with 1.0 ul denatured DNA; 1.0 ul 10 mM dNTPs; 2.0 ul        Reaction Buffer provided; 0.5 ul 32 mM SAM; DNMT1 [20 units];        0.3 ul 10× Enzyme 2 dilution and 1.0 ul 20× Enzyme 2 dilution,        replenishing with water. Incubate reactions at 37° C. for 5        hours, followed by heat-inactivate DNMT1 and Phi29 polymerase        for 20 minutes at 65° C., and hold reactions at 4° C. after        completion.        -   (1) Bisulfite treatment and Sanger sequencing to detect 5mC            patterns of differentially methylated genes after 5mC-WGA            amplification of pico grams of human gDNA.

In this example, the efficiency and accuracy of genomic 5mCamplification by 5mC-WGA are evaluated.

Example 2 Bisulfite Treatment of 5mC-WGA Product

Bisulfite treatment is carried out with commercial kits, for exampleusing EZ DNA Methylation-Direct™ Kit from Zymo. 20 ul of 5mC-WGA productis mixed with freshly prepared CT Conversion Reagent solution in a PCRtube, boiled at 98° C. for 8 minutes, followed by incubation at 64° C.for 3.5 hours, then returned to 4° C. The reaction is then purifiedusing the standard column provided in the kit. Note that at this stepthe products can be quantified using Qubit ssDNA kit (ThermoFisher). Forinput <50 pg the typical amplification can be over 1,000 fold.

Example 3 Detect 5mC Patterns of Differentially Methylated Genes bySanger Sequencing

The purified bisulfite converted product is further subjected to PCRamplification of specific genomic regions using hot start DNApolymerase, for example ZymoTaq™ DNA Polymerase from Zymo. Examplegenomic regions, their methylation patterns and bisulfite-specificprimer designs are shown in FIG. 2. The sequence of the primers arelisted below.

NFATC1-For TTTTTTGTAATAAGAGGAAGTATAGTTTTA NFATC1-RevATCTCCCAAATCCAAACTACTATC (hypermethylated) MAPK8IP2-ForGGTTGTGTAGTTTTTATTGAGTGTTTA MAPK8IP2-Rev AATCCCCCCAAAAACCCTAAC(unmethylated)

1-4 ul converted product is used as template for PCR amplification,accompanied by 25 ul ZymoTaq™ PreMix, 5 ul gene specific primer mix (10uM), and nuclease-free water up to 50 ul total volume. The PCR reactionis carried out with the following protocol: Initial denaturation at 95°C. for 10 min, then a cycle of denaturation at 95° C. for 30 sec,annealing at 55° C. for 30 sec, extension at 72° C. for 60 sec for atotal of 40-50 cycles, and a final extension step at 72° C. for 7 minfollowed by 4° C. holding for >4 min. Completed PCR is then purifiedusing either spin column-based or magnetic beads-based clean upprotocol. The purified products are analyzed with Sanger sequencingusing corresponding forward primers. Example results are shown in FIG.3. Sequence reads as ‘C’ indicate 5mC methylated sites, reads as ‘T’indicate unmethylated sites, while reads as ‘N’ or ‘Y’ indicate 5mCsites that are not completely maintained after amplification, or a denovo site added by DNMT1.

Example 4 Study of the Optimal Enzymatic Ratio of DNMT1 and Phi29 andInput Amount

To optimize the coupled enzymatic reactions of DNMT1 and Phi29,different ratios of the two enzymes are used to perform the 5mC-WGAreaction, with the efficiency evaluated by bisulfite treatment andSanger sequencing described above. 10 ng of human brain gDNA is used asthe input sample, different enzyme ratios ranging from 1:4 to 1:15 areused, with rest of experimental steps identical from the previouslydescribed protocol. The results are shown in FIG. 4a . The resultsindicate Phi29:DNMT1=1:15 as the optimal enzyme ratio.

To evaluate the versatility of 5mC-WGA for different input DNA amounts,5 pg-10 ng human brain gDNA are used in 5mC-WGA with the optimal enzymeratio. The results are shown in FIG. 4b . The results indicate 5mC-WGAis suitable for different input DNA amounts from 10 ng (and above) to 5pg (amount of DNA from a single cell) with stable performance.

(2) Using Other Forms of Biological Samples for 5mC-WGA.

The applicability of other forms of biological samples to be useddirectly for 5mC-WGA amplification is discussed in this example. Morespecifically, the option of using cells directly as input sample for5mC-WGA, and the option of using cell-free DNA extracted from bodyfluids (e.g. blood) as input sample for 5mC-WGA, are demonstrated.

Example 5 Use Cell-Free DNA as 5mC-WGA Input Sample

Cell-free DNA extracted from body fluids is generally present at verylow abundance and partially degraded, causing major technicaldifficulties to detect its methylation patterns which contain valuablediagnostic information. The applicability of using cell-free DNAdirectly as 5mC-WGA input sample is demonstrated. First, a control groupusing 1 ng mouse gDNA sonicated to ˜300 bp is used as the input samplefor 5mC-WGA reaction to ensure the efficiency of amplification offragmented DNA. The product is purified and analyzed using Bioanalyzer(Agilent), with result showing efficient amplification (FIG. 5a ). Next,100 pg of cell-free DNA extracted from pancreatic cancer patient blood(˜200 bp) is used in the 5mC-WGA reaction. The purified product isanalyzed with Bioanalyzer (Agilent). The profile shown in FIG. 5bresembles the sonicated gDNA profile, which indicates cell-free DNA canbe directly used as input sample of 5mC-WGA for efficient amplification.

Example 6 Use Cells as 5mC-WGA Input Sample

In addition to using purified DNA as the input sample for 5mC-WGA,processed cells can be used directly in 5mC-WGA, skipping extrapurification steps and facilitating low cell number or single celldetection applications.

Extracted cells need to be first lysed before being added into thereaction. The compatible lysis protocol is described here: Prepare twobuffers used in the lysis protocol, buffer L (400 mM KOH, 10 mM EDTA,100 mM DTT) and buffer N (200 mM HCl, 300 mM Tris pH7.5). To theextracted cells (1-15 cells, scale up for more cells), add 5 ul bufferL, incubate on ice for 10 min. Then add 5 ul buffer N, mix well. Thelysate can now be added into a 5mC-WGA reaction for amplification of DNAand 5mC patterns.

(3) MeDIP-Seq of Pico Grams of Human Brain gDNA Using 5mC-WGA Method

DNA amplified using 5mC-WGA can be further analyzed with high-throughputsequencing to reveal genome-wide 5mC patterns, either in clusters (byMeDIP-seq) or at single-base resolution (by Whole-Genome BisulfiteSequencing, WGBS).

For MeDIP-seq, the purified DNA can be sonicated and subjected to theprocessing with commercial MeDIP and DNA NGS library construction kits.Here we use KAPA HyperPlus and ThermoFisher MethylMiner kits as anexample to demonstrate the workflow.

Example 7 Sample Preparation

100 pg human brain gDNA is used as the input sample for 5mC-WGAamplification according to the protocol described previously. Theproduct is purified and the concentration is measured with Nanodrop.Both amplified product and template DNA (as positive control) are usedfor MeDIP-seq library construction.

Example 8 DNA Processing Before MeDIP

Example use of KAPA HyperPlus kit for pre-MeDIP processing of DNA.

DNA fragmentation: 100 ng purified 5mC-WGA product and template DNA aresubjected to enzymatic fragmentation according to manufacturer'sprotocol. 14 ul DNA is mixed with 2 ul fragmentation buffer and 4 ulfragmentation enzyme mix, incubated at 37° C. for 30 min, then thereaction is quenched on ice.

End repair and A-tailling: 20 ul mixture from last step is supplementedwith 2.8 ul end repair buffer and 1.2 ul enzyme mix, incubated at 65° C.for 30 min, then left at room temperature for 5-10 min for betterrepairing.

Adaptor ligation: 24 ul mixture from last step is supplemented with 1.2ul index (25 uM, Bioo), 12 ul ligation buffer, 4 ul ligase, andnuclease-free water up to 44 ul. The reaction is kept at 20° C. for 60min, followed by Ampure beads purification with 1:1 volume ratio. Theprocessed DNA is now ready to be enriched with MeDIP.

Example 9 5mC DNA Enrichment with MeDIP

Example use of ThermoFisher MethylMiner kit for MeDIP enrichment ofmethylated DNA fragments.

Follow the manufacturer's protocol, first prepare the magnetic beads andcouple the MBD-biotin protein. Then incubate MBD-beads with processedDNA generated from the previous steps, using the protocol for 5 ng-1 μginput DNA. Finally remove the uncaptured DNA and elute the captured,5mC-containing DNA fragments. These enriched products are now ready forlibrary amplification.

Example 10 MeDIP-Seq Library Amplification

Example use of KAPA HyperPlus kit for post-MeDIP amplification of DNAlibrary.

qPCR of the enriched DNA fragments: To determine the accurate number ofPCR cycles needed for amplifying the MeDIP library, 1 ul of template DNAor MeDIP enriched DNA is used in a 20 ul qPCR reaction containing 10 ulqPCR master mix (e.g. Roche FastStart SYBR Green) and 2 ul primer mix.The cycle number generating enough library is determined and used in thesubsequent PCR reaction.

Amplification of MeDIP libraries: Based on the cycle numbers determinedby qPCR, a 50 ul reaction containing template or enriched DNA, 25 ulKAPA HiFi HotStartReadyMix, and 5 ul primer mix is prepared andamplified in a thermal cycler. The products are further purified andsubjected to NGS-sequencing.

The resulting data demonstrate good maintenance of genome-wide 5mCpatterns using 5mC-WGS. A few example regions are shown in FIG. 6.

(4) WGBS of 5mC-WGA Amplified DNA from Limited Biological Samples.

For WGBS, the purified DNA is first subjected to bisulfite treatment,followed by processing using commercial WGBS library construction kits.Here we use Illumina TruSeq DNA Methylation kit as an example todemonstrate the workflow.

Example 11 Bisulfite Treatment of 5mC-WGA Product

Same with the protocol described in the first example. Note EZ DNAMethylation-Gold and EZ DNA Methylation-Lightning kits from Zymo can beused as alternative options.

Example 12 WGBS Library Construction

Example use of Illumina TruSeq DNA Methylation kit for WGBS libraryconstruction from bisulfite treated 5mC-WGS products.

Follow the manufacturer's protocol, first anneal the DNA synthesisadaptors to the denatured ssDNA, then synthesize complementary DNAstrand to produce dsDNA product, next tag the dsDNA with known adaptorsequences at both ends, finally amplify the library with specificindexes sequences for multiplexed NGS-sequencing.

Example 13 5mC-Retained Amplification of Specific Loci from LimitedBiological Samples

The reaction for specific loci with methylated sites retained is onlydifferent from the one for whole genome amplification with the additionof 4 ul both forward and reverse primers (10 nM) of the region ofinterest. Example use is the amplification of the FMR1 gene as apotential diagnosis of Fragile X Syndrome. Corresponding primers are:

FMR1_Forward GCTCAGCTCCGTTTCGGTTTCACTTCCGGT FMR1_ReverseCCTCCATCTTCTCTTCAGCCCTThe reaction is prepared also with 8 ul primer pairs (each one at 5 nM)and incubated based on the same thermocycle.

Preferred embodiments of this invention are described herein, includingappropriate examples known to the inventors for the application of thisinvention. The applications described above are exemplary only, andshould not be considered as limiting the scope of this invention.

REFERENCES

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1. A kit comprising, in one or more suitable containers, a stranddisplacement polymerase, DNMT1, S-adenosine methionine, dNTPs, randomprimers, and one or more nucleic acid buffers.
 2. The kit of claim 1,wherein the strand displacement polymerase is Phi29.
 3. The kit of claim1, wherein the kit further comprises an enzyme with a primase and apolymerase dual activity to synthesize DNA primers.
 4. The kit of claim3, wherein the enzyme is Thermus thermophilus PrimPol.
 5. The kit ofclaim 1, further comprising bovine serum albumin (BSA).
 6. The kit ofclaim 1, further comprising magnesium.
 7. The kit of claim 1, whereinthe strand displacement polymerase and the DNMT1 are at a ratio ofbetween about 1:5 units and about 1:20 units.
 8. The kit of claim 7,wherein the strand displacement polymerase and the DNMT1 are at a ratioof about 1:5 units.
 9. The kit of claim 7, wherein the stranddisplacement polymerase and the DNMT1 are at a ratio of about 1:10units.
 10. The kit of claim 7, wherein the strand displacementpolymerase and the DNMT1 are at a ratio of about 1:15 units.
 11. The kitof claim 7, wherein the strand displacement polymerase and the DNMT1 areat a ratio of about 1:20 units.
 12. The kit of claim 1, furthercomprising instructions for incubating the strand displacementpolymerase and the DNMT1 with methylated genomic DNA at a ratio ofpolymerase units to DNMT1 units between 1:5 units and 1:20 units. 13.The kit of claim 12, wherein the instructions are for incubating thestrand displacement polymerase and the DNMT1 with methylated genomic DNAat a ratio of polymerase units to DNMT1 units of 1:5 units.
 14. The kitof claim 12, wherein the instructions are for incubating the stranddisplacement polymerase and the DNMT1 with methylated genomic DNA at aratio of polymerase units to DNMT1 units of 1:10 units.
 15. The kit ofclaim 12, wherein the instructions are for incubating the stranddisplacement polymerase and the DNMT1 with methylated genomic DNA at aratio of polymerase units to DNMT1 units of 1:15 units.
 16. The kit ofclaim 12, wherein the instructions are for incubating the stranddisplacement polymerase and the DNMT1 with methylated genomic DNA at aratio of polymerase units to DNMT1 units of 1:20 units.